|
Clinical Interface |
|||||||||||||
|
|
|||||||||||||
|
The Clinical Interface module of the Ophthalmology Core Services provides isolation and temporary storage of cell pellets from human biological samples, including whole blood, mouthwash and buccal swabs. This service also offers the isolation and purification of high quality, high molecular weight, genomic DNA from these samples. |
|||||||||||||
|
Location:
Samples can be submitted on any weekday.
Please bring samples to the lab before Prior to bringing samples to the lab for
processing for the first time, please contact Dr. Wiggs to discuss details
of the type of processing required for your samples (cell pellet preparation or
DNA extraction or both). Module
personnel can assist in the development of special protocols required for
your study and this should be discussed with Dr. Wiggs prior to the
initiation of sample collection. Module
personnel can also assist with the collection of samples from patients
located a distance from the Massachusetts Eye and Ear Infirmary. Please discuss these plans with Dr. Wiggs
prior to collection. Whole blood is to be collected via venipuncture into lavender-topped venous blood collection
tubes (for example Becton-Dickenson cat# 364300 or 366643). These tubes
contain K2 EDTA as a preservative. All tubes of blood
must be clearly labeled with a date and either a patient name, study
identifier, or both. MOUTHWASH COLLECTION
(swish and spit): Mouthwash
collection kits can be obtained from the module personnel with instructions
for sample collection. Cytobrushes for cheek swabbing can be obtained from the module personnel with
instructions for sample collection.
For buccal swab samples DNA is prepared
directly rather than from an isolated cell pellet. If cell pellets are desired please contact
Dr. Wiggs. All samples should be brought to Dr. Wiggs’
laboratory located on the 7th floor of the connecting building of
Massachusetts Eye and Ear Infirmary, Blood samples must be transported to the
lab in an approved biohazard container. If the sample is transported
from outside the hospital it must be packaged according to IATA instructions
for the shipment of diagnostic samples. In brief, samples must be contained
within a watertight primary container (the blood tube) which is within a
watertight secondary container filled with enough absorbent material to
absorb the entire contents of the blood tubes. This secondary container must
then be housed in a third outer container of adequate strength for its
capacity. COLLECTION OF BLOOD SAMPLES DRAWN AT
THE MEEI 8TH FLOOR CLINICAL LABS: If you anticipate frequent use of the
phlebotomy services at the MEEI clinical lab (8th floor) for a large number
of samples for your project, you can arrange to keep a collection box
for your samples in the clinical lab and members of our lab will pick up
these samples for you on a daily basis. The placement of this box must
be arranged with the head of the clinical lab in advance. If you want
to utilize this service please make sure that the box is clearly labeled with
the name of the principal investigator of the study and that you have
notified members of our lab. For bloods collected on a sporadic basis,
we advise that you arrange to bring the samples to our lab yourself since lab
members of the clinical lab may not be familiar with what to do with your
samples. The Clinical Interface lab has space for
only temporary storage of samples from each user lab. Arrangements must
be made to retrieve your samples frequently. Nuclear pellets will be
stored in a specified labeled box on a designated shelf of a -80°C
freezer. Each investigator's samples will be stored in a separate
box. To retrieve samples bring a container filled with dry ice and
transfer your samples out of the designated box and return the empty box to
the shelf. DNA samples will also be temporarily
stored in a box designated for each investigator.
|
|||||||||||||